Distribution of apolipoprotein A-IV in human plasma.
نویسندگان
چکیده
منابع مشابه
Distribution of apolipoprotein A-IV in human plasma.
Human apoA-IV was purified from delipidated urinary chylomicrons. Monospecific antibodies were raised in rabbits and used to develop a double antibody radioimmunoassay (RIA). Displacement of 125I-labeled apoA-IV by plasma or purified chylomicron apoA-IV resulted in parallel displacement curves, indicating that apoA-IV from both sources share common antigenic determinants. The apoA-IV level in p...
متن کاملHuman apolipoprotein A-IV. Intestinal origin and distribution in plasma.
The role of the human intestine has been explored as a site of synthesis of apoA-IV, a major apoprotein of human intestinal triglyceride-rich lipoproteins. Intestinal biopsies were performed on normal volunteers while fasting and after lipid ingestion. Indirect immunofluorescence demonstrated a marked increase in immunofluorescence for apoA-IV during lipid absorption consistent with an increase...
متن کاملQuantification of apolipoprotein A-IV in human plasma by immunonephelometry.
We developed a nephelometric procedure for determining concentrations of human plasma apolipoprotein (apo) A-IV. Results obtained correlate well with those measured by an established electroimmunodiffusion assay (r = 0.98; n = 56). Intra- and interassay CVs were 2.4% and 2.0%, respectively, indicating excellent precision and reproducibility. Various conditions of sample treatment, such as adequ...
متن کاملCharacterization of apolipoprotein A-IV complexes and A-IV isoforms in human lymph and plasma lipoproteins.
We have isolated and characterised A-IV apolipoprotein (apo-A-IV) from human lymph and plasma by immunoabsorbance chromatography and two-dimensional electrophoresis. Two different apo-A-IV-containing lipoproteins were isolated from four different sources, human lymph triglyceride-rich fraction (TRL), lymph lipoprotein-deficient fraction (LDF), plasma high-density lipoprotein (HDL), and plasma l...
متن کاملDifferential distribution of apolipoprotein E isoforms in human plasma lipoproteins.
The polymorphism of apolipoprotein E (apo E) accounts for a substantial amount of the genetic variance of cholesterol levels in man. The epsilon-2 allele and the epsilon-4 allele raises plasma and low density lipoprotein cholesterol levels as compared to the epsilon-3 allele. Whereas the lower cholesterol levels in carriers of the epsilon-2 allele can, at least in part, be attributed to the gro...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1985
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)34400-x